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KMID : 0382619900100020771
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Hanyang Journal of Medicine
1990 Volume.10 No. 2 p.771 ~ p.777
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Polyacrylamide Gel Electrophoresis (PAGE) for Detection of Unusual Group A, and Non-Group A Human Rotaviruses (Rv)
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Abstract
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Viral RNA analysis by PAGE, a sensitive and highly specific test for detection Rv in stool, was used to classify human Group A Rv into subgroups Ⅰand Ⅱand to find non-group A human Rv. Of 151 fecal samples positive for group A-specific monoclonal antibody(Mab)-based ELISA, 27(18%) belonged to subgroup Ⅰ(short elec-tropherotype)and 119(79%)were identified as subgroup Ⅱ(long electropherotype).
Two samples contained mixed infections of the two electropherotypes. One isolate contained atypically migrating RNA with two extra segments between segment 4 and 5. Another isolate belong to neither one of the two electropherotypes, but they did.not yield enough viral RNA to allow their classification. It is possible that reassort-ment viruses have emerged as a result of the patients´being infected simultaneously with belonging to the two electropherotypes. The unusual strains are being adapted to MA 104 cells and cultured for further antigenic analysis.
PAGE yidlded RNA typical of group A Rv in 5 Mab-based ELISA-negative stools, and thus raised the sensitivity of the ELISA.
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